Validation of suitable endogenous control genes for quantitative PCR analysis of microRNA gene expression in a rat model of endometrial cancer
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comparison of snrna-u6 and microrna-16 for identification of suitable endogenous control gene for microrna gene expression analysis under dendrosomal curcumin treatment in hepatocellular carcinoma cell lines
objective: micrornas (mirnas) are single-stranded small rnas 18-25 nucleotides in length that regulate gene expression through translational inhibition and mrna cleavage. aberrant expression of mirnas contribute to several diseases. this has increased interest in profiling the expressions of these molecules. real-time quantitative pcr (rq-pcr) is a sensitive, quantitative technique for gene exp...
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15 صفحه اولValidation of suitable endogenous control genes for expression studies of miRNA in prostate cancer tissues.
When performing quantitative polymerase chain reaction analysis, there is a need for correction of technical variation between experiments. This correction is most commonly performed by using endogenous control genes, which are stably expressed across samples, as reference genes for normal expression in a specific tissue. In microRNA (miRNA) studies, two types of control genes are commonly used...
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ژورنال
عنوان ژورنال: Cancer Cell International
سال: 2013
ISSN: 1475-2867
DOI: 10.1186/1475-2867-13-45